The purpose of this study was to isolate DNA probe which could identify Fusobacterium nucleatum strains Southern blot analysis. This study procedure includes (1) whole-genomic DNA extraction of Fusobacterium nucleatum (F. nucleatum) ATCC 25586, F. nucleatum ATCC 23726, F. nucleatum ATCC 10953, F. nucleatum ATCC 49256, F. nucleatum ATCC 51190 (2) construction of the genomic DNA library, (3) screening of restriction fragment of genomic DNA, (4) identification of species-specific DNA probe by Southern blot hybridization. About 150 restriction fragments of several F. nucleatum genomic DNA digested with the Hind Ⅲwere obtained. One of them, the probe Fu9, restriction fragments of F. nucleatum Fusobacterium nucleatum ATCC 25586, was shown to be hybridized with all of the F. nucleatum strains used in this study but the length of the bands are different among the strains. From that result, the probe Fu9 may be used in identification of F. nucleatum strains.
|